Theta Signal Transfer from Parietal to Prefrontal Cortex Ignites Conscious Awareness of Implicit Knowledge during Sequence Learning.

Unconscious acquisition of sequence structure from experienced events can lead to explicit awareness of the pattern through extended practice. Although the implicit-to-explicit transition has been extensively studied in humans using the serial reaction time (SRT) task, the subtle neural activity supporting this transition remains unclear. Here, we investigated whether frequency-specific neural signal transfer contributes to this transition. A total of 208 participants (107 females) learned a sequence pattern through a multisession SRT task, allowing us to observe the transitions. Session-by-session measures of participants' awareness for sequence knowledge were conducted during the SRT task to identify the session when the transition occurred. By analyzing time course RT data using switchpoint modeling, we identified an increase in learning benefit specifically at the transition session. Electroencephalogram (EEG)/magnetoencephalogram (MEG) recordings revealed increased theta power in parietal (precuneus) regions one session before the transition (pretransition) and a prefrontal (superior frontal gyrus; SFG) one at the transition session. Phase transfer entropy (PTE) analysis confirmed that directional theta transfer from precuneus → SFG occurred at the pretransition session and its strength positively predicted learning improvement at the subsequent transition session. Furthermore, repetitive transcranial magnetic stimulation (TMS) modulated precuneus theta power and altered transfer strength from precuneus to SFG, resulting in changes in both transition rate and learning benefit at that specific point of transition. Our brain-stimulation evidence supports a role for parietal → prefrontal theta signal transfer in igniting conscious awareness of implicitly acquired knowledge.SIGNIFICANCE STATEMENT There exists a pervasive phenomenon wherein individuals unconsciously acquire sequence patterns from their environment, gradually becoming aware of the underlying regularities through repeated practice. While previous studies have established the robustness of this implicit-to-explicit transition in humans, the refined neural mechanisms facilitating conscious access to implicit knowledge remain poorly understood. Here, we demonstrate that prefrontal activity, known to be crucial for conscious awareness, is triggered by neural signal transfer originating from the posterior brain region, specifically the precuneus. By employing brain stimulation techniques, we establish a causal link between neural signal transfer and the occurrence of awareness. Our findings unveil a mechanism by which implicit knowledge becomes consciously accessible in human cognition.

Neural dynamics underlying the processing of implicit form-meaning connections: The dissociative roles of theta and alpha oscillations.

Implicit learning plays an important role in the language acquisition. In addition to helping people acquire the form-level rules (e.g., the word order regularities), implicit learning can also facilitate the acquisition of word meanings (i.e., the establishment of connections between the word form and its meanings). Although some behavioral studies have explored the processing of implicit form-meaning connections, the neural dynamics underlying this processing remains unclear. Through examining whether participants could implicitly acquire the literal and metaphorical meanings of novel words, and applying the time-frequency analysis on the electroencephalogram (EEG) data collected in the testing phase, the neural oscillations corresponding to the processing of implicit form-literal and form-metaphorical meaning connections were explored. The results showed that participants in the experimental group could implicitly acquire the form-literal and form-metaphorical meaning connections after training, while participants in the control group who were not trained did not have access to such form-meaning connections. Meanwhile, during the processing of form-literal meaning connections, the greater suppression of alpha oscillations was induced by the testing items that follow the same rules as the training items (i.e., the regular testing items) in the experimental group, whereas the stronger enhancement of theta oscillations was elicited by the regular testing items in the experimental group during the processing of form-metaphorical meaning connections. Our study provides insights for understanding the processing of implicit form-literal and form-metaphorical meaning connections and the neural dynamics underlying the processing.